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primescript rt master mix reverse transcription kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript rt master mix reverse transcription kit
    Primescript Rt Master Mix Reverse Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+master+mix+reverse+transcription+kit/PrimeScript+RT+Master+Mix/pm42098781-84-13-20
    Average 99 stars, based on 26473 article reviews
    primescript rt master mix reverse transcription kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: SPATA22 heterozygous variants drive premature ovarian insufficiency: mechanism of granulosa cells dysfunction and therapeutic potential of N-acetylcysteine (NAC) treatment.
    Article Snippet: Total RNA was extracted using the MiniBEST Universal RNA Extraction Kit (Takara, Japan) following the manufacturer’s protocol. .. Reverse transcription was performed AR TIC LE IN PR ES S using the PrimeScript RT Master Mix Reverse Transcription Kit (Takara) to synthesize complementary DNA (cDNA). .. RT-qPCR was performed using TB Green® Premix Ex TaqTM II (Takara) on an Applied Biosystems Real-Time Quantitative PCR System (Thermo Fisher Scientific, USA).

    Article Title: 3D printed flexible composite scaffold with ultrasonic-driven wireless electrical stimulation promotes neuro-vascularization for critical-size bone defects regeneration
    Article Snippet: .. RNA was reverse-transcribed to cDNA using the PrimeScript RT Master Mix reverse transcription kit (Takara Bio Inc., Otsu, Shiga, Japan) according to the manufacturer's instructions. ..

    Article Title: PGE 2 Modulates Uterine Luminal Fluid Composition and Endometrial Function in Dairy Heifers During Diestrus.
    Article Snippet: Total RNA was extracted from the bEECs using TRIzol reagent (Takara, Tokyo, Japan) according to the manufacturer’s instructions. .. RNA concentration and purity were determined spectrophotometrically, and total RNA (1 μg) was reverse-transcribed into complementary DNA (cDNA) using the PrimeScript RT Master Mix reverse transcription kit (RR036, Takara). .. Real-time quantitative PCR (qPCR) was performed using SYBR Green Master Mix (Q311, Vazyme, Nanjing, China) on a Bio-Rad CFX96 system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) following the manufacturer’s protocol.

    Article Title: <p>KMO downregulation promotes hepatocellular carcinoma growth via 3‑HAA‑mediated mitochondrial mass and function imbalances</p>.
    Article Snippet: Total RNA was extracted from tissue samples or cultured cells using RNAiso Plus (Takara Bio, Inc.). .. RNA was reverse‐transcribed into cDNA using the PrimeScript RT Master Mix Reverse Transcription Kit (Takara Bio, Inc.). .. The target gene expression was quantified by two‐step qPCR using TB Green® Premix Ex Taq II (Takara Bio, Inc.) with the proce‐ dure: pre‐denaturation at 95 ̊C for 30 sec, 1 cycle; denatured at 95 ̊C for 5 sec, annealed at 60 ̊C and extended for 30 sec, 40 cycles.

    Article Title: Sunflower HaGLK Enhances Photosynthesis, Grain Yields, and Stress Tolerance of Rice
    Article Snippet: .. Total RNA of sunflower leaves was extracted using TRIzol reagent (Thermo, San Jose, CA, USA) and reverse transcribed to full-length cDNA by the PrimeScript RT Master Mix reverse transcription kit (TaKaRa, Hangzhou, China). ..

    Article Title: KMO downregulation promotes hepatocellular carcinoma growth via 3-HAA-mediated mitochondrial mass and function imbalances
    Article Snippet: Total RNA was extracted from tissue samples or cultured cells using RNAiso Plus (Takara Bio, Inc.). .. RNA was reverse-transcribed into cDNA using the PrimeScript RT Master Mix Reverse Transcription Kit (Takara Bio, Inc.). .. The target gene expression was quantified by two-step qPCR using TB Green ® Premix Ex Taq II (Takara Bio, Inc.) with the procedure: pre-denaturation at 95°C for 30 sec, 1 cycle; denatured at 95°C for 5 sec, annealed at 60°C and extended for 30 sec, 40 cycles.

    Article Title: Sunflower HaGLK Enhances Photosynthesis, Grain Yields, and Stress Tolerance of Rice
    Article Snippet: .. Total RNA of leaves from the positive transgenic plants was extracted using TRIzol reagent (Thermo, San Jose, CA, USA) and reverse transcribed to full-length cDNA by the PrimeScript RT Master Mix reverse transcription kit (TaKaRa, Hangzhou, China) according to the manufacturer’s instructions. .. Quantitative real-time PCR (qPCR) was performed using the SYBR Green Master Mix kit (TOYOBO, Guangzhou, China).

    Article Title: PGE 2 Modulates Uterine Luminal Fluid Composition and Endometrial Function in Dairy Heifers During Diestrus
    Article Snippet: Total RNA was extracted from the bEECs using TRIzol reagent (Takara, Tokyo, Japan) according to the manufacturer’s instructions. .. RNA concentration and purity were determined spectrophotometrically, and total RNA (1 μg) was reverse-transcribed into complementary DNA (cDNA) using the PrimeScript RT Master Mix reverse transcription kit (RR036, Takara). .. Real-time quantitative PCR (qPCR) was performed using SYBR Green Master Mix (Q311, Vazyme, Nanjing, China) on a Bio-Rad CFX96 system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) following the manufacturer’s protocol.

    Concentration Assay:

    Article Title: PGE 2 Modulates Uterine Luminal Fluid Composition and Endometrial Function in Dairy Heifers During Diestrus.
    Article Snippet: Total RNA was extracted from the bEECs using TRIzol reagent (Takara, Tokyo, Japan) according to the manufacturer’s instructions. .. RNA concentration and purity were determined spectrophotometrically, and total RNA (1 μg) was reverse-transcribed into complementary DNA (cDNA) using the PrimeScript RT Master Mix reverse transcription kit (RR036, Takara). .. Real-time quantitative PCR (qPCR) was performed using SYBR Green Master Mix (Q311, Vazyme, Nanjing, China) on a Bio-Rad CFX96 system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) following the manufacturer’s protocol.

    Article Title: PGE 2 Modulates Uterine Luminal Fluid Composition and Endometrial Function in Dairy Heifers During Diestrus
    Article Snippet: Total RNA was extracted from the bEECs using TRIzol reagent (Takara, Tokyo, Japan) according to the manufacturer’s instructions. .. RNA concentration and purity were determined spectrophotometrically, and total RNA (1 μg) was reverse-transcribed into complementary DNA (cDNA) using the PrimeScript RT Master Mix reverse transcription kit (RR036, Takara). .. Real-time quantitative PCR (qPCR) was performed using SYBR Green Master Mix (Q311, Vazyme, Nanjing, China) on a Bio-Rad CFX96 system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) following the manufacturer’s protocol.

    Transgenic Assay:

    Article Title: Sunflower HaGLK Enhances Photosynthesis, Grain Yields, and Stress Tolerance of Rice
    Article Snippet: .. Total RNA of leaves from the positive transgenic plants was extracted using TRIzol reagent (Thermo, San Jose, CA, USA) and reverse transcribed to full-length cDNA by the PrimeScript RT Master Mix reverse transcription kit (TaKaRa, Hangzhou, China) according to the manufacturer’s instructions. .. Quantitative real-time PCR (qPCR) was performed using the SYBR Green Master Mix kit (TOYOBO, Guangzhou, China).



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